AKTIVITASANTIBAKTERI SAPONIN HASIL ISOLASI ALOE BARBADENSISMILLERTERHADAPSTAPHYLOCOCCUSAUREUS PENYEBAB MASTITIS PADA SAPI PERAH

Imbang Dwi Rahayu


Abstract


The mastitis in dairy cattle is caused by Staphylococcus aureus. The bacteria are multiresistant
to antibiotic. Isolated of Aloe barbadensis Miller, that has antibacterial, saponin is needed to replace
synthetic antibiotic. The research has conducted to tested antibacterial activity of saponin for the
growth of Staphylococcus aureus.
Saponin was exstracted from gel of Aloe vera leafs by n-hexane and methanol. Isolated of
saponin used to the Coulom Cromatography and Thin Layer Cromatography. It needs H2SO4 for
identifying of saponin. The saponin’s powder was produced by drying process, amylum or dexstrin
as kinds of filler. The ratio of saponin and amylum or dexstrin was 80% : 20%. Six levels of saponin
were used in experiment i.e : 1,5%; 3,0%; 6,2%; 12,5%; 25% and 50%. The parameters of the
research were Minimum Inhibition Concentration (MIC) and cakram disk. The result of experiment
was analysed with descriptively, penicillin was used as positive control.
The saponin’s powder give antibacterial activity that higher than saponin’s concentrated. There
were similarity in antibacterial activity between the saponin’s powder was added amylum with
penicillin.

Keywords


Key word :Saponin, antibacterial activity, Aloe barbadensis Miller, Staphylococcus aureus.

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