In Vitro Rumen Fermentation Parameter and Gas Production Rice Straw Fermented With Lignochloritic Innoculum

Indah Prihartini S.Chuzaemi O. Sofjan


Abstract


Background: Lignin degradation in rumen is very limited and only a few rumen microbes able to transform uncondensize or aromatic form into another hidrolyzable compound. The research was conducted to study rumen fermentation product from fermented rice straw (FRS) with lignochloritik isolates.

Metode: Isolates type LC1 (TLiD). LC2 (BopR) as first factor and incubation duration (0,3,7,12 dan 18 days) were used in this experiment, and replicated thrice. In vitro gas production was measured at 0, 4, 8, 16, 24, 48, 72, and 96 hours incubation. The parameter included (a) gas production at 0 hours, (b) gas production potency, and (c) gas production rate that were measured by  NAWAY programe. At the of fermentatio, supernatanr used for rumen VFA and NH3.

Hasil:  Gas production increase along incubation time. The highest gas production was reached at 96 hours incubation for control (63,167 ml/500 mg BK). FRS gas production lower than control, but the organic matter degradation higher than control.  Isolates gave highly signficant (P<0.01) influence into VFA and NH3. Interaction isolates type and incubation duration has significant (P<0.05) effect into VFA dan NH3 production. Acetic, propionic, and butyric production with LC2 higher than LC1 and LC3. On the other hand, NH3 production with LC3 higher   LC2 and LC3.   Fermentation with lignochloritik in rice straw increased synthesis microbes. The highest VFA and NH3 production were reached at third days with BOpR and TLiD.


Keywords


Fermentation; gas production; in vitro; inoculum; lignochloritik; VFA; NH3; umm

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