EFEK ISOFLAVON FITOESTROGEN DARI EKSTRAK Pueraria lobata TERHADAP MEMORI DAN AKTIVITAS KOLINERGIK DI HIPPOKAMPUS CA1 PADA TIKUS HIPOESTROGEN

Abstract

Data from retrospective and case-conttrole showed that estrogen replacement therapy (ERT) could prevent ang delayed against
Alzheimer Disease (AD). The using of ERT is still controversial because of their long-term side effect. Isoflavon as a part of phytoestrogen
has potential effect as an alternative substitute for ERT. The aim of this study is to examine the effect of phytoestrogen from Pueraria lobata
extract in increasing memory’s function and whether it occur by the increased of cholinergic activity in CA1 hippocampus. Exploration study
performed by three step. First, determined genistein and daidzein concentration in Pueraria lobata extract, as basic of dose determining.
Second, oophorectomy performed to made a hypoestrogenic rat’s model; and third, determined method memory’s function in rats. This study
used twenty five female Rattus novergicus strain Wistar, 10 -11 weeks of age, five rats were normal rats (N) as first group and twenty rats
were hypoestrogenic rats for 2nd, 3rd, 4th, and 5th groups. All of the hypoestrogenic rat were devided into 4 groups, each 5 rats. Hypoestrogenic
rats group without Isoflavon Genistein Daidzein supplement (OVX), hypoestrogenic rats group with Isoflavon Genistein Daidzein
supplement (OVX+IGD) dosage of 15, 30, and 60 mg/kgweight/day each. The IGD was given for 21 days. The Morris Water Morris
(MWM) test was performed in 17th, 18th, 19th, 20th, and 21st day. On 22nd day rats were killed. Frozen section of the brain was performed
immediately. Cholinergic activities on CA1 hippocampus were examined with AChE staining. A significant difference was found on
memory’s function and cholinergic activities on CA1 hippocampus between OVX and OVX+IGD groups at all doses (p value = 0,000 of
each). No significant difference on IGD effect toward brain weight (p=0,936) and pyramidal cell number on CA1 hippocampus (p=0,961).
It was suggest that increased memory’s function on OVX+IGD groups were caused by the increased of cholinergic activities on CA1
hippocampus.
Key word : Isoflavones; phytoestrogen; Genistein; Daidzein; Pueraria lobata; Memory; cholinergic.