Pengembangan Teknologi Produksi Khamir rhodotorula sp. Sebagai Agensia Pengendali Hayati Penyakit Antraknosa pada Cabai

Abstract

Pengembangan Teknologi Produksi Khamir rhodotorula sp. Sebagai  Agensia Pengendali Hayati Penyakit Antraknosa pada Cabai

Dian Indratmi

Staf Pengajar Jurusan Agronomi, Fakultas Pertanian dan Peternakan
Universitas Muhammadiyah Malang
e-mail: indratmi_dian@yahoo.co.id

ABSTRACT

The aimed of this research to find out a low cost media culture formulation for yeast fructoplant Rhodotorula  sp. biomass production for their application on field to suppressed anthracnose disease on pepper.
The  laboratory  experiment  with  Complete  Randomized  Design  and  3 replication. There were 10 treatment, it’s M1 (rain tree pod extract + potato extract); M2 (rain tree pod extract + chilli extract ); M3 (corn meal+ chilli extract); M4 (sugar potato extract); M5 (sugar chilli extract); M6 (potato  dextrose broth); M7 (corn solid media); M8 (100% chilli extract); M9 (sugar corn meal); dan M10 (100% rain tree pod extract) Results of the experiment indicated from 10 of the yeast media culture test   100%
Chilli extract medium and rain tree pod extract + potato extract medium resulted better spore weight and cell density of  Rhodotorula sp. yeast. Its shown with spore weight  0.4833 g/ 10 ml to 100% chilli extract  and cell density 59.67 x 103  cell/ ml. At rain tree pod extract + potato extract medium, spore weight  0.4845 g / 10 ml with cell density 70.67x103  cell/ml. Production Rhodotorula sp. yeasy cell on solid medium can used corn medium.

Key word : Colletrotichum gloeosporioides, Rhodotorula sp. , Biocontrol