ISOLASI DAN IDENTIFIKASI SAPONIN DARI ALOE BARBADENSIS MILLER SEBAGAI ANTIBIOTIK ALAMI : PENANGGULANGAN MASTITIS PADA SAPI PERAH

Abstract

The mastitis in dairy cattle is caused by Staphylococcus aureus. The bacteria are multiresistant
to antibiotic. Isolated of Aloe barbadensis Miller, that has antibacterial, saponin is needed to replace
synthetic antibiotic. The research has conducted to tested antibacterial activity of saponin for the
growth of Staphylococcus aureus.
Saponin was exstracted from gel of Aloe vera leafs by 95% ethanol, 50% ethanol and nbutanol.
Isolated of saponin used to the Coulom Cromatography and Thin Layer Cromatography. It
needs “Liebermann-Burchad” reagent, acetic anhidryde and sulfuric acid (1:1:1:1) for identifying
of saponin. These saponin preparation were detected by short wave U.V lamp on silica gel G plates
developed with solvent of butanol-ethanol-water (2 : 4: 4). The saponin’s powder was produced by
drying process, amylum or dexstrin as kinds of filler. The ratio of saponin and dexstrin was 80% :
20%. Six levels of saponin were used in experiment i.e : 1,5%; 3,0%; 6,2%; 12,5%; 25% and 50%.
The parameters of the research were Minimum Inhibition Concentration (MIC) and cakram disk.
The result of experiment was analysed with descriptively, penicillin was used as positive control.
The saponin’s powder give antibacterial activity that higher than saponin’s concentrated. There
were similarity in antibacterial activity between the saponin’s powder was added dextrin with
penicillin.