AKTIVITAS ANTIMIKROBA EKSTRAK LIDAH BUAYA (ALOE BARBADENSIS MILLER) TERHADAP BAKTERI PATOGEN PADA IKAN

Abstract

“Streptocciacis” disease caused by streptococcus agalactiac has been attacked many tilapia
fish farming, with the symptoms including abdominal bloating; bleeding in the eye, gill cover and
base of tail; become darker in color, and fast swmming fish unpalatable. Antibiotcs have been used
to over come this diseases, but the use of antibiotics continuously and unwisely can lead to bacterial
resistence and impact on the envirotment, therefore alternative method for controlling the disease
is important. this study was animed study was to detemine the antimicrobial activity of Aloe
barbadensisi extract (saponin) toward S.agalactiae. Fresh Aloe barbandensis leaf washed then
sterilized by using alcohol, dired in a preheated oven, then percolated and maserated by using n
hexane and methanol for 24 hours. Then performed gravity column chromatography with a BEA
developer (Benzene Ethyl Acetate,Acetic acid with the ratio 75: 24: 1) on silica gel plates to ontain
saponin, then dried with a vacumm freeze dryer by using a dextrin as carrier agent, to from powder.
MIC test and Inhibition zone test were performed to find the antimicrobial activity of sopanin
extracted from Aloe barbadensis Miller, againt S.agalactiae. The result showed that the higher the
concentration of saponin extracted from Aloe barbadensis Miller, the better the atimicrobial activity
againt S.agalactiane. The highest of inhition zone found at concentration 12,5% with a doameter
zone of 8.97 mm.