Effectiveness Test Combination Of Roselle Flower (Hibiscus Sabdariffa L) And Green Tea (Camellia Sinensis) Extract With Vancomycin Antibiotic Against Methicillin Resistant Staphylococcus Aureus (Mrsa) Bacteria In Vitro

Abstract

Background: MRSA is a strain of Staphylococcus aureus bacteria that is resistant to methicillin antibiotics. These bacteria can cause various infections of the skin, soft tissue, Etc. The incidence of resistance to MRSA is currently increasing significantly, including in Indonesia. Inappropriate use of vancomycin as MRSA therapy makes resistance to Staphylococcus aureus bacteria and causes a high risk of complications. Purpose: This research aims to compare the inhibitory effect between the combination of roselle flower extract (Hibiscus sabdariffa L) and green tea leaf (Camellia sinensis) with vancomycin antibiotics against MRSA bacteria. Methods: True experimental: post-test only control design, with the Kirby Bauer disc diffusion method with extract concentrations of 100%, 50%, 25%, and 12.5%, which will then be determined the Minimum Inhibitory Content (MIC). Statistical data analysis used One Way ANOVA and Post Hoc Bonferroni tests, and descriptive analysis used the Greenwood classification (1995). Results: MIC combination of roselle and green tea extract against MRSA bacteria’s growth was 12.5%. One Way ANOVA test showed significant differences (p=0.000) in the inhibition zone of the combination of roselle and green tea extract against MRSA bacteria growth. Post-Hoc Bonferroni test showed no significant difference (p=1,000) between roselle and green tea extract at a concentration of 12,5% and vancomycin 30mg, and there were significant differences (p=0,000) at a concentration of 100%, 50%, and 25% against MRSA bacteria growth and vancomycin 30mg. Conclusion: The combination of roselle and green tea extract has a stronger sensitivity than vancomycin antibiotics to MRSA bacteria’s in vitro