Hypoxic Status Is Associated With The Intensity Of Hypoxia Inducible Factor (Hif)-1α Expression In A Premature Placenta
Keywords:Premature, placenta, hypoxia, HIF-1α
Prematurity refers to live births before 37 weeks of gestation and associated with infant morbidity/mortality. Activation of HIF during the final pregnancy phase is believed to play a critical role in the pathogenesis of premature birth and other pregnancy disorders. This study aimed to analyze the relationship between hypoxicstatus and the intensity of HIF-1α expression in a premature placenta.Stored biological materials premature placenta (paraffin blocks) was used in this study. Thirtyone samples of placental hypoxia (H) and 28 samples of premature placental non-hypoxia (N) as controls, were selected non-random consecutively. Subsequently, immunohistochemistry was performed to analyze HIF-1α expression. TheChi-square testwas used to analyze the data and a p-value <0.05 was considered statistically significant.Moderate to strong intensity of HIF-1α expressionwas observed in 58% of hypoxic placenta samples, whereas most of non-hypoxic placental samples(86%) did not expressed or expressed weaklyHIF-1α.There was a significant correlation between the intensity of HIF-1α expression and placental hypoxia (p <0.05) and Odds Ratio (OR) value was 8.31 with a 95% confidence interval (2.32-29.77). The conclusion shows that hypoxic status is associated with intensity of hypoxia inducible factor (HIF)-1α expression in a premature placenta.
Abe, H. et al. (2013) ‘Nitric Oxide Induces Vascular Endothelial Growth Factor Expression in the Rat Placenta in Vivo and in Vitro’, Bioscience Biotechnology and Biochemistry, 77(5), pp. 971–6. doi: 10.1271/bbb.120923.
Ahumada-Barrios, M. E. and Alvarado, G. F. (2016) ‘Risk Factors for premature birth in a hospital’, Revista Latino-Americana de Enfermagem, 24, pp. 1–8. doi: 10.1590/1518-8345.0775.2750.
Andraweera, P. H. et al. (2012) ‘Placental expression of VEGF family mRNA in adverse pregnancy outcomes’, Placenta, 33, pp. 467–72. doi: 10.1016/j.placenta.2012.02.013.
Chakraborty, D., et al. (2016) ‘HIF-KDM3A-MMP12 regulatory circuit ensures trophoblast plasticity and placental adaptations to hypoxia’, PNAS, 113(46), pp. E7212–E7221. doi: https://doi.org/10.1073/pnas.1612626113.
Dong, X. et al. (2012) ‘The expression of thioredoxin-1 in preterm delivery placenta’, Redox Report, 17(5), pp. 187–93. doi: 10.1179/1351000212y.0000000021.
García-Honduvilla, N. et al. (2018) ‘Placentas from women with pregnancy-associated venous insufficiency show villi damage with evidence of hypoxic cellular stress’, Human Pathology, 77, pp. 45–53. doi: 10.1016/j.humpath.2018.03.022.
Gopagondanahalli, K. R. et al. (2016) ‘Preterm Hypoxic–Ischemic Encephalopathy’, Frontiers in Pediatrics, 4(114), pp. 1–10. doi: 10.3389/fped.2016.00114.
Horii, M. et al. (2016) ‘Human pluripotent stem cells as a model of trophoblast differentiation in both normal development and disease’, Proceedings of the National Academy of Sciences of the United States of America, 113(27), pp. 3882–91. doi: 10.1073/pnas.1604747113.
Jellema, R. K. et al. (2013) ‘Cerebral inflammation and mobilization of the peripheral immune system following global hypoxia-ischemia in preterm sheep’, Journal of Neuroinflammation, 10(13), pp. 1–19. doi: 10.1186/1742-2094-10-13.
Kartini, K. et al. (2015) ‘Fetal blood vessel count increases in compensation of hypoxia in premature placentas’, UNIVERSA MEDICINA, 34(1), pp. 35–42. doi: 10.18051/univmed.2015.v34.035.
Kockar, F. (2012) ‘Hypoxia and cytokines regulate carbonic anhydrase 9 expression in hepatocellular carcinoma cells in vitro’, World Journal of Clinical Oncology, 3(6), pp. 82–91. doi: 10.5306/wjco.v3.i6.82.
Lai, H. and Liu, H. (2018) ‘Expression and meaning analysis of HIF-1α and HSP70 in preeclamptic placenta’, Biomedical Research (India), 29(6), pp. 1240–43. doi: 10.4066/biomedicalresearch.29-17-3632.
Liu, W., Wang, S. J. and Lin, Q. De (2014) ‘Study on the expressions of PHD and HIF in placentas from normal pregnant women and patients with preeclampsia’, International Journal of Biological Sciences, 10(3), pp. 278–84. doi: 10.7150/ijbs.6375.
Masoud, G. N. and Li, W. (2015) ‘HIF-1α pathway: Role, regulation and intervention for cancer therapy’, Acta Pharmaceutica Sinica B, pp. 378–89. doi: 10.1016/j.apsb.2015.05.007.
Ortega, M. A. et al. (2019) ‘Upregulation of VEGF and PEDF in placentas of women with lower extremity venous insufficiency during pregnancy and its implication in villous calcification’, BioMed Research International, 2019, p. 5320902. doi: 10.1155/2019/5320902.
Patel, J. et al. (2010) ‘Regulation of Hypoxia Inducible Factors (HIF) in hypoxia and normoxia during placental development’, Placenta, 31(11), pp. 951–957. doi: 10.1016/j.placenta.2010.08.008.
Rath, G. et al. (2016) ‘HIF-1 Alpha and Placental Growth Factor in pregnancies complicated with preeclampsia: A qualitative and quantitative analysis’, Journal of Clinical Laboratory Analysis, 30(1), pp. 75–83. doi: 10.1002/jcla.21819.
Ravarino, A. et al. (2014) ‘Cerebral hypoxia and ischemia in preterm infants the role of the clinical pathological dialogue in problem solving’, Proceedings Journal of Pediatric and Neonatal Individualized Medicine •, 3(2), pp. 1–9. doi: 10.7363/030272.
Robb, K. P. et al. (2017) ‘Inflammation-induced fetal growth restriction in rats is associated with increased placental HIF-1α accumulation’, PLoS ONE, 12(4), pp. 1–15. doi: 10.1371/journal.pone.0175805.
Salmani, D. et al. (2014) ‘Study of structural changes in placenta in pregnancy-induced hypertension.’, Journal of natural science, biology, and medicine, 5(2), pp. 352–355. doi: 10.4103/0976-9668.136182.
Sriyanti, R. et al. (2019) ‘The difference in maternal serum hypoxia-inducible factors-1α levels between early onset and late-onset preeclampsia’, Open Access Macedonian Journal of Medical Sciences, 7(13), pp. 2133–7. doi: 10.3889/oamjms.2019.601.
Sulistiarini, D. and Berliana, M. (2016) ‘Faktor-faktor yang memengaruhi kelahiran prematur di Indonesia : analisis data Riskesdas 2013’, E-Journal Widya Kesehatan dan Lingkungan, 1(2), pp. 109–115. doi: 10.1289/ehp.1002285.
Vadlapatla, R. K., Vadlapudi, A. D. and Mitra, A. K. (2013) ‘Hypoxia-inducible factor-1 (HIF-1): a potential target for intervention in ocular neovascular diseases’, Curr Drug Targets, 14(8), pp. 919–935. doi: 10.2174/13894501113149990015.
Yuan, D. et al. (2014) ‘Enhancement of the HIF-1α/15-LO/15-HETE axis promotes hypoxia-induced endothelial proliferation in preeclamptic pregnancy’, PLoS ONE, 9(5), p. e96510. doi: 10.1371/journal.pone.0096510.
Zaramella, P. et al. (2017) ‘Hypoxia-Inducible Factor in cord blood of term and preterm newborns’, J Ped Moth Care, 2(2), pp. 1–7.
Copyright (c) 2021 Kartini Edwin
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-ShareAlike 4.0 International License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.