Antimicrobial potential of Kaffir Lime (Citrus hystrix D.C) peel extract against Staphylococcus aureus

Authors

  • Ginda Nabila Choirunnisa Universitas Muhammadiyah Semarang
  • Kanti Ratnaningrum Universitas Muhammadiyah Semarang
  • Ika Dyah Kurniati Universitas Muhammadiyah Semarang

DOI:

https://doi.org/10.22219/sm.Vol18.SMUMM1.21112

Abstract

Staphylococcus aureus is a normal bacterial flora that can cause skin infections such as boils, acne, impetigo and is also a major cause of nosocomial infections. This study examined the effect of kaffir lime (Citrus hystrix D.C) peel extract on Staphylococcus aureus. This research was a true experimental study with a post test only control group design. Kaffir lime peel was extracted using maceration method with 70% ethanol solvent, then diluted using 2ml ethanol to a concentration of 25mg/ml, 20mg/ml, 15mg/ml, 10mg/ml and 5mg/ml. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were conducted to assess the kaffir lime peel extract against Staphylococcus aureus bacteria. The MIC test was measured using a spectrophotometer in the liquid dilution method and MBC test was carried out using solid dilution on Mueller Hinton media. Minimum Inhibitory Concentration of kaffir lime peel extract against Staphylococcus aureus bacteria at a concentration of 20mg / ml., while MBC in this study could not be determined because at a concentration of 25% there was still colony growth in repetitions 1 and 2.

Downloads

Download data is not yet available.

References

Davenport, Fm. Hennessy, AV. Bernstein, Sh. (2015) Comparative Incidence Of Influenza A-Prime In 1953 In Completely Vaccinated And Unvaccinated Military Groups. Am J Public Health. Vol 45: 1138–1146.

Fatimah S, Nadifah F, Burhanudin I. (2016) Uji Daya Hambat Ekstrak Etanol Kubis (Brassica Oleracea Var. Capitata F. Alba) Terhadap Bakteri Staphylococcus Aureus Secara In Vitro. Vol 4: 102–106.

Fenty S. (2013) Pola Kuman Dan Sensitivitas Antimikroba Pada Infeksi Saluran Kemih Fakultas Farmasi, Universitas Sanata Dharma, Yogyakarta. J Farm Sains Dan Komunitas; 10: 9–13.

Habibi Ai, Firmansyah Ra, Setyawati Sm.(2018)Skrining Fitokimia Ekstrak N -Heksan Korteks Batang Salam (Syzygium Polyanthum). Inonesian J Chem Sci. 7: 1–4.

Hombing, Yb. (2015) Efek Antibakteri Ekstrak Kulit Jeruk Purut (Citrus Hystrix D.C.) Terhadap Pertumbuhan Bakteri Porphyromonas Gingivalis Secara In Vitro.

Iqbal M, Putra H, Suwarto S. (2014) Faktor Risiko Methicillin Resistant Staphylococcus Aureus Pada Pasien Infeksi Kulit Dan Jaringan Lunak Di Ruang Rawat Inap. J Penyakit Dalam Indonesia. 1: 3–4.

Razak A, Aziz D, Gusti Revilla. (2013) Penelitian Uji Daya Hambat Air Perasan Buah Jeruk Nipis (Citrus Aurantifolia S.) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus Secara In Vitro. J Kesehat Andalas; 2: 5–8.

Rambet Lg, Waworuntu O, Gunawan Pn. (2017) Uji Konsentrasi Hambat Minimum ( Khm ) Perasan Murni Bawang Putih ( Allium Sativum ) Terhadap Pertumbuhan Candida Albicans. Pharmacon. 6: 16–23.

Saifuddin F H. (2018) Uji Konsentrasi Hambat Minimal Daun Jeruk Purut (Citrus Hystrix) Terhadap Pertumbuhan Staphylococcus Aureus Pada Ikan Bandeng (Chanos Chanos) (Studi In Vitro). Pros Semin Nas. 6: 16–23.

Setiawati A. (2015) Peningkatan Resistensi Kultur Bakteri Staphylococcus Aureus Terhadap Amoxicillin Menggunakan Metode Adaptif Gradual. J Farm Indone; 7: 190–194.

Tanjaya A, Hadinugroho W. (2019) Formulasi Tablet Hisap Ekstrak Etanol 96% Kulit Jeruk Purut (Citrus Hystrix) Sebagai Antiseptik Mulut. Farmaka; 15: 1–8.

Downloads

Published

2022-07-14

Issue

Vol. 18 No. 1 (2022): June 2022

Section

Article

License

Copyright (c) 2022 Kanti Ratnaningrum, Ginda Nabila Choirunnisa, Ika Dyah Kurniati

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-ShareAlike 4.0 International License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).


 Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.