PENGARUH PEMBERIAN EKSTRAK ETANOL DAUN KEMANGI (Ocimum basilicum L.) TERHADAP MOTILITAS SPERMATOZOA TIKUS PUTIH GALUR WISTAR JANTAN (Rattus norvegicus) YANG DIINDUKSI MONOSODIUM GLUTAMATE (MSG)
DOI:
https://doi.org/10.22219/sm.Vol14.SMUMM1.6241Abstract
Latar Belakang : MSG merupakan substansi kristal putih yang digunakan untuk meningkatkan rasa pada makanan. Pemberian MSG dosis tinggi meningkatkan kadar radikal bebas dalam tubuh yang menyebabkan penurunan motilitas spermatozoa. Kandungan flavonoid pada daun kemangi dapat menetralkan kadar radikal bebas sehingga mencegah penurunan motilitas spermatozoa. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak kemangi (Ocimum basilicum L.) dalam mencegah penurunan motilitas spermatozoa pada tikus putih jantan galur wistar yang diinduksi MSG.
Metode : Penelitian Laboratory Experimental Post Test Group Only menggunakan 24 ekor tikus putih galur wistar jantan (Rattus norvegicus) yang dibagi menjadi 4 kelompok, terdiri dari kelompok I diberi MSG 1.600 mg/kgBB/hari, kelompok II diberi MSG 1.600 mg/kgBB/hari dan ekstrak etanol daun kemangi 150mg/kgBB/hari, kelompok III diberi MSG 1.600 mg/kgBB/hari dan ekstrak etanol daun kemangi 350 mg/kgBB/hari dan kelompok IV diberi MSG 1.600 mg/kgBB/hari dan ekstrak etanol daun kemangi 700 mg/kgBB/hari. Data dianalisis menggunakan One Way ANOVA dan post hoc LSD
Hasil : Rerata presentase motilitas spermatozoa pada kelompok I, II, III dan IV sebesar 21,88%; 48,17%; 53,72%; dan 63,05%. Hasil analisis One Way Anova menunjukan adanya perbedaan signifikan antar kelompok (p = 0,000). Uji post hoc LSD menunjukan kelompok II, III dan IV berbeda nyata dengan kelompok I (p=0,00). Pada kelompok II dan III juga ditemukan adanya perbedaan nyata dengan kelompok IV (p=0,04). Sedangkan kelompok III dengan kelompok II tidak terdapat perbedaan nyata (p=0,069).
Kesimpulan : Ekstrak etanol daun kemangi (Ocimum basilicum L.) memberikan pengaruh dalam mencegah penurunan presentase motilitas spermatozoa tikus putih galur wistar jantan (Rattus norvegicus) yang diinduksi MSG.
Kata kunci : motilitas spermatozoa, ekstrak etanol daun kemangi, monosodium glutamat (MSG)
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