Phytochemical screening and determination of total phenolic and total flavonoid contents of pear peel extracts
DOI:
https://doi.org/10.22219/farmasains.v4i2.10166Keywords:
Phytochemicals, Phenolics, Flavonoids, Pear peels, AntioxidantAbstract
The study was carried out to investigate the presence of different phytochemicals as well as to determine the total phenolic and flavonoid contents of green pear/european pear peels and yellow pear/asian pear peels. Phytochemical screening of the pear peels was carried out following standard protocols. Total phenolic content was determined by Folin-Ciocalteu method and total flavonoid contents was determined by Aluminum Chloride Colorimetric method, measured using n-hexane, etil acetate, and methanol. Phenolics, flavonoids, tannins, alkaloids, and saponins are present in green pear and yellow pear peels. The total phenol and flavonoid contents are highest in methanol extracts in which green pear peels have a higher concentration than yellow pear peels. In conclusion, the green and yellow pear peels are rich in phenolic compounds and have great potential as natural antioxidants. Therefore, the results of this research should be considered to provide the possibility for future developments of highly functional antioxidants.
Downloads
References
Ajila, C. M., Naidu, K. A., Bhat, S. G., and Prasada, J. S. (2007). Bioactive Compounds and Antioxidant Potential of Mango Peel Extract. Food Chemistry, 105 (3): 982–88. https://doi.org/10.1016/j.foodchem.2007.04.052.
An, S., Ling P. Z., Shen, L. J., Wang, S., Kuo Zhang, K., Qi, Y., Zheng, J. (2017). USP18 Protects against Hepatic Steatosis and Insulin Resistance through Its Deubiquitinating Activity. Hepatology, 66 (6): 1866–84. https://doi.org/10.1002/hep.29375.
Ayoola, G.A., H.A.B. Coker, S.A. Adesegun, A.A. Adepoju-Bello, K. Obaweya, E.C. Ezennia, and T.O. Atangbayil. (2008). Phytochemical Screening and Antioxidant Activities of Some Selected Medicinal Plants Used for Malaria Therapy in South Western Nigeria. J. Pharm. Res., 7: 1019–24.
Borges, G., Mullen, W., and Crozier, A. (2010). Comparison of the Polyphenolic Composition and Antioxidant Activity of European Commercial Fruit Juices. Food and Function, 1 (1): 73–83. https://doi.org/10.1039/c0fo00008f.
Chang, C., Yang, M., Wen, H., Chern, J. (2002). Estimation of Total Flavonoid Content in Propolis by Two Complementary Colorimetric Methods. Journal of Food and Drug Analysis, 10 (3): 178–82.
Cui, T., Nakamura, K., Ma, L., Zhong, J.L., and Kayahara, H. (2005). Analyses of Arbutin and Chlorogenic Acid, the Major Phenolic Constituents in Oriental Pear. Journal of Agricultural and Food Chemistry, 53 (10): 3882–87. https://doi.org/10.1021/jf047878k.
Lesellier, E., Destandau, C., Grigoras, L., Fougère, C., Elfakir. (2012). Fast Separation of Triterpenoids by Supercritical Fluid Chromatography/Evaporative Light Scattering Detector. Journal of Chromatography A, 1268: 157–65. https://doi.org///doi.org/10.1016/j.chroma.2012.09.102.
Elfalleh, W. (2012). Total Phenolic Contents and Antioxidant Activities of Pomegranate Peel, Seed, Leaf and Flower. Journal of Medicinal Plants Research, 6 (32): 4724–30. https://doi.org/10.5897/jmpr11.995.
Ferrandiz, M.L., Alcaraz, M.J. (1991). Anti-Inflammatory Activity and Inhibition of Arachidonic Acid Metabolism by Flavonoids. Agents Actions, 32 (3–4): 283–288.
Ferreira, D., Guyot, S., Marnet, N., Delgadillo, I., Catherine M.G., Renard, C., and Coimbra, M A., (2002). Composition of Phenolic Compounds in a Portuguese Pear (Pyrus Communis L. Var. S. Bartolomeu) and Changes after Sun-Drying. Journal of Agricultural and Food Chemistry, 50 (16): 4537–44. https://doi.org/10.1021/jf020251m.
Gil, B., Sanz, M.J., Terencio, M.C., Ferrandiz, M.L., Bustos, G., Paya, M., Gunasegaran, R., Alcaraz, M.J. (1994). Accelerated Communication: Effects of Flavonoids on Naja Naja and Human Recombinant Synovial Phospholipases A2 and Inflammatory Responses in Mice.” Life Sciences, 54 (20): 333–38. https://doi.org///doi.org/10.1016/0024-3205(94)90021-3.
Halliwell, B. (1994). " Free radicals, antioxidants, and human disease: curiosity, cause, or consequence?” FREE RADICALS AND ANTIOXIDANTS, 344: 721–24. Nikhil, K. (2006). Medicinal Plants: Future Source of Pesticides. Asian Journal of Microbiology, Biotechnology and Environmental Sciences, 8 (1): 57–59. https://doi.org/10.13140/RG.2.1.1395.6085.
Kahle, K., Preston, C., Richling, E., Heckel, F., and Schreier, P. (2005). On-Line Gas Chromatography Combustion/Pyrolysis Isotope Ratio Mass Spectrometry (HRGC-C/P-IRMS) of Major Volatiles from Pear Fruit (Pyrus Communis) and Pear Products. Food Chemistry, 91 (3): 449–55. https://doi.org/10.1016/j.foodchem.2004.06.026.
Lee, Ki Hoon, Jeong Yong Cho, Hyoung Jae Lee, Young Kyu Ma, Joseph Kwon, Seong Hwa Park, Sang Hyun Lee, et al. (2011). “Hydroxycinnamoylmalic Acids and Their Methyl Esters from Pear (Pyrus Pyrifolia Nakai) Fruit Peel.” Journal of Agricultural and Food Chemistry, 59 (18): 10124–28. https://doi.org/10.1021/jf2022868.
Lee, Ki Hoon, Jeong Yong Cho, Hyoung Jae Lee, Keun Young Park, Young Kyu Ma, Sang Hyun Lee, Jeong An Cho, Wol Soo Kim, Keun Hyung Park, and Jae Hak Moon. (2011). “Isolation and Identification of Phenolic Compounds from an Asian Pear (Pyrus Pyrifolia Nakai) Fruit Peel.” Food Science and Biotechnology, 20 (6): 1539–45. https://doi.org/10.1007/s10068-011-0213-4.
Ma, Jian Nan, Su Le Wang, Ke Zhang, Zhi Gang Wu, Masao Hattori, Gui Lin Chen, and Chao Mei Ma. (2012). “Chemical Components and Antioxidant Activity of the Peels of Commercial Apple-Shaped Pear (Fruit of Pyrus Pyrifolia Cv. Pingguoli).” Journal of Food Science, 77 (10). https://doi.org/10.1111/j.1750-3841.2012.02899.x.
Martins, S., Mussatto, S. I., Guillermo M. A., Julio M. S., Aguilar, C. N., and Teixeira, J. A. (2011). Bioactive Phenolic Compounds: Production and Extraction by Solid-State Fermentation. A Review. Biotechnology Advances, 29 (3): 365–73. https://doi.org/10.1016/j.biotechadv.2011.01.008.
Pourmorad, F., Hosseinimehr, S J., and Shahabimajd, N. (2006). Antioxidant Activity, Phenol and Flavonoid Contents of Some Selected Iranian Medicinal Plants. African Journal of Biotechnology, 5 (11): 1142–45. https://doi.org/10.1055/s-2007-987042.
Tiwari, P., Kumar, B., Kaur, M., Kaur, G., Kaur, H. (2011). Phytochemical Screening and Extraction: A Review. Internationale Pharmaceutica Scienca, 1 (1).
Nandita, S., and Rajini, P. S. (2004). Free Radical Scavenging Activity of An Aqueous Extract of Potato Peel. Food CHemistry, 85 (May): 611–16. https://doi.org/10.1016/S0308-8146(03)00396-0.
Tanriöven, D., and Aziz E. (2005). Phenolic Compounds in Pear Juice from Different Cultivars. Food Chemistry, 93 (1): 89–93. https://doi.org/10.1016/j.foodchem.2004.09.009.
Terao, J., Piskula, M., Yao, Q. (1994). Protective Effect of Epicatechin, Epicatechin Gallate, and Quercetin on Lipid Peroxidation in Phospholipid Bilayers. Archives of Biochemistry and Biophysics, 308 (1): 278–84. https://doi.org///doi.org/10.1006/abbi.1994.1039
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2020 Vinda Maharani Patricia, Fauzia Ningrum Saputri
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
a. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).