Antidiabetic activities of 96% ethanol extract of Abelmoschus esculentus on Balb-C mice strain
DOI:
https://doi.org/10.22219/farmasains.v4i2.10718Keywords:
Diabetes mellitus, Balb-c strain mice, Abelmoschus esculentus, AntioxidantsAbstract
Diabetes mellitus (DM) is a progressive metabolic disease that continues to increase every year. Treatment therapy using oral hypoglycemic drugs to treat DM has side effects, and alternative approaches must be taken. Abelmoschus esculentus has been known to be used as an alternative to DM because it has antioxidant activity due to its high flavonoid content. The purpose of this study was to determine the antidiabetic activity of 96% ethanol extract of Okra leaves against male mice of balb-c strain. Male mice were acclimatized for seven days and divided into five test groups and then fasted for 18 hours. Then the mice were induced with 150 mg / 20 g BW alloxan and measured on the 3rd day. Ethanol extract 96% okra leaves were then given in a dose of 5.6 mg / 20g BW; 11.2 mg / 20g BW; 22.4 mg / 20g BW, CMC Na 0.5% as negative control and glibenclamide 0.026 mg / 20g BBs as positive control for 14 days. The results of therapy with 96% ethanol extract of okra leaves can reduce blood glucose levels in mice so that the okra leaves contain flavonoid compounds that have antioxidant activity and can reduce blood glucose levels.
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Dipiro, J. T., Talbert, R. L., Yee, G. C., Matzke, G. R., Wells, B. G., & Posey, L. M. (Eds.). (2016). Pharmacotherapy: A Pathophysiologic Approach (10thed.). New York, NY: McGraw-Hill Medical.
Dubey, P., & Mishra, S. (2017). A Review on: Diabetes and Okra (Abelmoschus esculentus). Journal of Medicinal Plants Studies, 5(3), 23-26.
Durazzo, A., Lucarini, M., Novellino, E., Souto, E. B., Daliu, P., & Santini, A. (2019). Abelmoschus esculentus (L.): Bioactive Components’ Beneficial Properties—Focused on Antidiabetic Role—For Sustainable Health Applications. Molecules, 24(1), 38.
Idris, H., Hasyim, H., & Utama, F. (2017). Analysis of Diabetes Mellitus Determinants in Indonesia: A Study from the Indonesian Basic Health Resesarch 2013. Acta Medica Indonesiana, 49(4), 291-298.
Ighodaro, O. M., Adeosun, A. M., & Akinloye, O. A. (2017). Alloxan-induced Diabetes, A Common Model for Evaluating the Glycemic-control Potential of Therapeutic Compounds and Plants Extract in Experimental Studies. Medicina, 53(6), 365-374.
Kumar, A., Ilavarasan, R., Jayachandran, T., Deecaraman, M., Aravindan, P., Padmanabhan, N., & Krishan, M. R. V. (2008). Anti-diabetic activity of Syzygium cumini and its isolated compound against streptozotocin-induced diabetic rats. Journal of Medicinal Plants Research, 2(9), 246-249.
Kumar, D. S., Tony, D. E., Kumar, A. P., Kumar, K. A., Srinivasa, D. B., & Nadendla, R. (2013). A Review On: Abelmoschus esculentus (Okra). International Research Journal of Pharmaceutical and Applied Sciences, 3(4), 129-132.
Lailiyah, I. Y. (2016). Uji Aktivitas Antidiabetes Ekstrak Etanol 96% Kulit Pisang Mas (Musa acuminata Colla) pada Mencit Jantan Galur Babl-C (Undergraduate Thesis). Akademi Farmasi Jember, Jember, Indonesia.
Lathifah, N. L. (2016). Hubungan Durasi Penyakit dan Kadar Gula Darah dengan Keluhan Subjektif Penderita Diabetes Mellitus. Jurnal Berkala Epidemiologi, 5(2), 231-239.
Lorenzati, B., Zucco, C., Miglietta, S., Lamberti, F., & Bruno, G. (2010). Oral Hypoglicemic Drugs: Pathophysiological Basis of Their Mechanism of Action. Pharmaceuticals, 3(9), 3005-3020.
Muhammad, I., Matazu, K. I., Yaradua, A. I., Nasir, A., Matazu, N. U., Zainab, A. S., ... & Abbas, A. Y. (2017). Antidiabetic Activity of Abelmoschus esculentus (Ex-Maradi Okra) Fruit in Alloxan-induced Diabetic Rats. Nigerian Journal of Biochemistry and Molecular Biology, 32(1), 44-52.
Mycek, M. J., Harvey R.A., & Champe P.C. (2001). Farmakologi Ulasan Bergambar. Jakarta, Indonesia: Widya Medika.
Pasaribu, F., Sitorus, P., & Bahri, S. (2012). Uji Ekstrak Etanol Kulit Buah Manggis (Garcinia Mangostana L.) Terhadap Penurunan Kadar Glukosa Darah. Journal of Pharmaceutics and Pharmacology, 1(1), 1-8.
Peng, C. H., Lin, H. C., Lin, C. L., Wang, C. J., & Huang, C. N. (2019). Abelmoschus esculentus subfractions improved nephropathy with regulating dipeptidyl peptidase-4 and type 1 glucagon-like peptide receptor in type 2 diabetic rats. Journal of Food and Drug Analysis, 27(1), 135-144.
Sangeetha, R. (2019). Luteolin in the Management of Type 2 Diabetes Mellitus. Current Research in Nutrition and Food Science, 7(2), 393-398. Simanjuntak, H. A. (2018).
Pemanfaatan Tumbuhan Obat Diabetes Mellitus di Masyarakat Etnis Simalungun Kabupaten Simalungun Provinsi Sumatera Utara. BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan), 5(1), 59-70.
Sindhu, R. K., & Puri, V. (2016). Phytochemical, Nutritional and Pharmacological Evidences for Abelmoschus esculentus (L.). The Journal of Phytopharmacology, 5(6), 238-241.
Sukandar, E. Y., Andrajati R., Sigit J. I., Adnyana I, K., Setiadi, A. A. P., Kusnandar. (2008). ISO Farmakoterapi. Jakarta, Indonesia: ISFI.
Szkudelski, T. (2001). The Mechanism of Alloxan and Streptozotocin Action in B Cells of the Rat Pancreas. Physiological Research, 50(6), 537-546.
Testa, R., Bonfigli, A. R., Genovese, S., de Nigris, V., & Ceriello, A. (2016). The Possible Role of Flavonoid in Prevention of Diabetic Complication. Nutrients, 8(5), 310.
Torkpo, S. K., Danquah, E. Y., Offei, S. K., & Blay, E. T. (2006). Esterase, total protein and seed storage protein diversity in Okra (Abelmoschus esculentus L. Moench). West African Journal of Applied Ecology, 9(1), 1-7.
Yuriska, F. (2009). Efek Aloksan Terhadap Kadar Glukosa Darah Tikus Wistar (Undergraduate Thesis). Universitas Diponegoro, Semarang, Indonesia.
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