Comparison of Antioxidant Activities of Ethyl Acetate Extracts of Two Varieties of Sweet Potato Tuber [Ipomoea Batatas (L.)] Using Two Extraction Methodsomoea batatas (L.)] MENGGUNAKAN DUA METODE EKSTRAKSI
DOI:
https://doi.org/10.22219/farmasains.v6i2.18069Keywords:
sweet potato, tuber, ipomoea batatas, antioxidantAbstract
The decrease in antioxidant activity can be caused by the destruction of antioxidant compounds due to heating. Several studies stated that there was no significant difference between the antioxidant activity of extracts from cold extraction and hot extraction. This study compares the antioxidant activity of the ethyl acetate extract of two varieties of sweet potato using two different extraction methods. Simplicia was extracted by hot extraction using reflux and cold extraction using maceration. The antioxidant activity of the extracts was tested by the DPPH method using UV-Vis spectrophotometry. There are two types of sweet potato used in this research; firstly, the outer skin of the tuber is purple, the inside is purple (UU), and, secondly, the outer skin is purple, the inside is orange (UO). The IC50 UUR (Purple-Purple Refluxed), UOR (Purple-Orange Refluxed), UUM (Purple-Purple Macerated), UOM (Purple-Orange Macerated) , and ascorbic acid values were 4.583, 4.614, 0.755, 18.142, and 2.680 g/ml; thus, the extraction method and sweet potato varieties affect the antioxidant activity of the extract. Maceration is the best method for UU, while reflux is the best method for UO.
Downloads
References
Dewi, I., S, M., & Verawaty. (2020). Perbandingan Metode Sokletasi dengan Maserasi terhadap Daya Aktivitas Antioksidan Bunga Tasbih (Canna hybrida Hort.). Jurnal Farmasi Higea, 12(1), 48–54.
Fadlilaturrahmah, Wathan, N., Firdaus, A., & Arishandi, S. (2020). Pengaruh Metode Ekstraksi Terhadap Aktivitas Antioksidan dan Kadar Flavonoid Daun Kareho (Callicarpa Longifolia Lam). Pharma Xplore, 5(1), 23–33.
Fidrianny, I., Suhendy, H., & Insanu, M. (2018). Correlation of phytochemical content with antioxidant potential of various sweet potato (Ipomoea batatas) in West Java, Indonesia. Asian Pacific Journal of Tropical Biomedicine, 8(1), 25. https://doi.org/10.4103/2221-1691.221131
Harborne, J. B. (1987). Metode Fitokimia. ITB.
Hartiati, A., Sri, M., & Made, D. P. (2009). Pengaruh preparasi bahan baku rosella dan waktu pemasakan terhadap aktivitas antioksidan sirup bunga rosella (Hisbiscus sabdariffa L.). Agrotekno, 15(1), 20–24.
Hasanah, M., Andriani, N., & Noprizon. (2016). Perbandingan Aktivitas Antioksidan Ekstrak Etanol Daun Kersen (Muntingia Calabura L.) Hasil Ekstraksi Maserasi dan Refluks. Scientia, 6(2), 84–90.
Jing, C., XF, D., & Tong, J. (2015). Optimization of Ultrasonic-Assisted Extraction of Flavonoid Compounds and Antioxidants from Alfalfa Using Response Surface Method. Molecules, 20.
Manggala, E., Purwanti, L., & Syafnir, L. (2017). Perbandingan Aktivitas Antioksidan Ekstrak Etanol Daun Mangga Bapang Mangifera Indica L.) dengan Metode Ekstraksi Maserasi dan Refluks.
Prosiding Farmasi, 67–71.
Mokoginta, E., Runtuwene, M., & Wehantouw, F. (2013). Pengaruh Metode Ekstraksi Terhadap
Aktivitas Penangkal Radikal Bebas Ekstrak Metanol Kulit Biji Pinang Yaki (Areca vestiaria Giseke). Pharmacon Jurnal Ilmiah Farmasi, 3(1), 109–113.
Molyneux, P. (2004). The Use Of The Stable Free Radical Diphenylpicrylhidrazyl (DPPH) For Estimating Antioxidant Activity. Songklanakarin J. Sci. Technol, 26(2), 211–219.
Nurhasnawati, H., & Handayani, S. (2017). Perbandingan Metode Ekstraksi Maserasi dan Sokletasi Terhadap Aktivitas Antioksidan Ekstrak Etanol Daun Jambu Bol (Syzygium Malaccense L.). Jurnal Ilmiah Manuntung, 3(1), 91–95.
Nurlaela, E., Sakti, E., & Sadiyah, E. (2016). . Pengaruh Perbedaan Metode Ekstraksi Terhadap Aktivitas Antioksidan Ekstrak Etanol dan Fraksi Daun Srigading (Nyctanthes Arbor-Tristis L.).
Prosiding Farmasi, 481–487.
Pu, F., Ren, X., & Zhang, X. (2013). Phenolic compounds and antioxidant activity in fruits of six Diospyros kaki genotypes. European Food Research and Technology, 237(6), 923–932.
Risnadewi, W., Muliasari, H., Hamdin, C., & Andayani, Y. (2019). Comparative antioxidant activity of Brucea javanica (L) Merr seed extract derived from maceration and soxhletation method. AIP Conference Proceedings, 1–7.
Saptarini, N., & Wardati, Y. (2020). Effect of Extraction Methods on Antioxidant Activity of Papery Skin Extracts and Fractions of Maja Cipanas Onion (Allium cepa L. var. ascalonicum) Hindaw. E Scientific World Journal.
Setyowati, W., & Damayanti, D. (2014). Pengaruh Metode Ekstraksi Terhadap Aktivitas Antioksidan Kulit Buah Durian (Durio zibethinus Murr) Varietas Petruk. Prosiding Seminar Nasional Pendidikan Sains.
Suhendy, H., Kusnadiawan, W., & DD, A. (2021). Pengaruh Metode Maserasi dan Refluks Terhadap Total Fenol Dan Flavonoid Dari Dua Varietas Umbi Ubi Jalar (Ipomoea Batatas L.). Pharmacoscript, 4(1), 98–108.
Sulastri, L., Oktavia, I., & Simanjuntak, P. (2020). Aktivitas Antioksidan Kecibeling, Bakau Merah, dan Katuk Pada Metode Ekstraksi dan Rasio Ekstrak Yang Berbeda. Buletin Penelitian Tanaman Rempah Dan Obat, 31(1), 1–7.
Verawati, Nofiandi, D., & Petmawati. (2017). Pengaruh Metode Ekstraksi Terhadap Kadar Fenolat Total dan Aktivitas Antioksidan Daun Salam (Syzygium polyanthum (Wight) Walp.). Jurnal Katalisator, 2(2), 53–60.
Yin, W., Duan, S., & Zhang, Y. (2010). . Antioxidant activities of different solvents extracts and alkaloids of Uncaria rhynchophylla (Miq.) Jacks. Journal of Guangxi Normal University, 28(1), 31–34.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Hendy Suhendy, Defri Risviana, Imas Ratnasari
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
a. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).