Effication of Viral Nervous Necrosis DNA as Vaccines for Cromileptes altivelis
Keywords:Antibody titer, DNA vaccine, Immunity, Survival rate
Viral nervous necrosis (VNN) is a disease that often infects groupers. It has caused mass death in more than 34 species of marine fish. DNA vaccination might become a solution againts the infection. The construction of the pmBA-CP DNA vaccine consisting of the beta-actin promoter of medaka fish (Oryzias latipes) and capsid protein (CP) encoding VNN RNA2 has been made in previous studies. This study aimed to test the efficacy of the pmBA-CP DNA vaccine for VNN. The experiment consisted of two stages, namely (1) detection of anti-VNN antibody induction in vaccinated fish using ELISA, and (2) challenge test for fish vaccinated with the VNN virus. Grouper (body length 8 cm to 10 cm) were divided into two groups with a density of 5 fish 60 L–1. The fish in the first group were vaccinated with pmBA-CP intramuscularly at a dose of 12.5 µg per fish, while the second group of fish were not vaccinated. Antibody titer testing was carried out before treatment, and 1 d, 7 d, 14 d, 21 d, 28 d, and 35 d after vaccination. The challenge test was carried out on the 60th day after vaccination. The results showed that the S / P ratio in the vaccinated fish serum was higher than unvaccinated fish at 21 d to 35 d post-vaccination. DNA vaccination was able to induce anti-VNN antibodies of grouper. The results of the challenge test for vaccinated fish using VNN virus titer 103.5 FID50/0.2 mL showed 60% of survival rate. Thus, the pmBA-CP DNA vaccine could be useful for increasing grouper immunity, and support production of grouper.
Anderson E D, Leong J-A C. 2000. Development of DNA vaccines for salmonid fish. In DNA Vaccines (pp. 105–121): Springer.
Chi S, Lo B, Lin S. 2001. Characterization of grouper nervous necrosis virus (GNNV). Journal of Fish Diseases 24(1): 3–13.https://doi.org/10.1046/j.1365-2761.2001.00256.x
Emmenegger E J, Kurath G. 2008. DNA vaccine protects ornamental koi (Cyprinus carpio koi) against North American spring viremia of carp virus. Vaccine 26(50): 6415–6421. https://doi.org/10.1016/j.vaccine.2008.08.071
Goidl E A, Paul W E, Siskind G W, Benacerraf B. 1968. The Effect of Antigen Dose and Time after Immunization on the Amount and Affinity of Anti-Hapten Antibody. The Journal of Immunology 100(2): 371–375.
Kai Y-H, Chi S-C. 2008. Efficacies of inactivated vaccines against betanodavirus in grouper larvae (Epinephelus coioides) by bath immunization. Vaccine 26(11): 1450–1457. https://doi.org/10.1016/j.vaccine.2007.12.043
Kim C H, Johnson M C, Drennan J D, Simon B E, Thomann E, Leong J A. 2000. DNA vaccines encoding viral glycoproteins induce nonspecific immunity and Mx protein synthesis in fish. Journal of virology 74(15): 7048–7054. 10.1128/jvi.74.15.7048-7054.2000
Kumar S R, Parameswaran V, Ahmed V P I, Musthaq S S, Hameed A S S. 2007. Protective efficiency of DNA vaccination in Asian seabass (Lates calcarifer) against Vibrio anguillarum. Fish & Shellfish Immunology 23(2): 316–326. https://doi.org/10.1016/j.fsi.2006.11.005
Lin C-C, Lin J H-Y, Chen M-S, Yang H-L. 2007. An oral nervous necrosis virus vaccine that induces protective immunity in larvae of grouper (Epinephelus coioides). Aquaculture 268(1): 265–273. https://doi.org/10.1016/j.aquaculture.2007.04.066
Lorenzen N, LaPatra S J R S E T-O I D E. 2005. DNA vaccines for aquacultured fish. 24(1): 201.
Nuryati S, Alimuddin A, Sukenda S, Soejoedono R D, Santika A, Pasaribu F H, Sumantadinata K. 2010. Construction of a DNA vaccine using glycoprotein gene and its expression towards increasing survival rate of KHV-infected common carp (Cyprinus carpio). Jurnal Natur Indonesia 13(1): 47–52.
Pakingking R, Bautista N B, de Jesus-Ayson E G, Reyes O. 2010. Protective immunity against viral nervous necrosis (VNN) in brown-marbled grouper (Epinephelus fuscogutattus) following vaccination with inactivated betanodavirus. Fish & Shellfish Immunology 28(4): 525–533. https://doi.org/10.1016/j.fsi.2009.12.004
Sambrook J, Fritsch E F, Maniatis T. 1989. Molecular cloning: a laboratory manual: Cold spring harbor laboratory press.
Sommerset I, Lorenzen E, Lorenzen N, Bleie H, Nerland A H. 2003. A DNA vaccine directed against a rainbow trout rhabdovirus induces early protection against a nodavirus challenge in turbot. Vaccine 21(32): 4661-4667. https://doi.org/10.1016/S0264-410X(03)00526-7
Takagi S, Sasado T, Tamiya G, Ozato K, Wakamatsu Y, Takeshita A, Kimura M. 1994. An efficient expression vector for transgenic medaka construction. Mol Mar Biol Biotechnol 3(4): 192–199.
Vazquez-Juarez R C, Gomez-Chiarri M, Barrera-Saldaña H, Hernandez-Saavedra N, Dumas S, Ascencio F. 2005. Evaluation of DNA vaccination of spotted sand bass (Paralabrax maculatofasciatus) with two major outer-membrane protein-encoding genes from Aeromonas veronii. Fish & Shellfish Immunology 19(2): 153–163. https://doi.org/10.1016/j.fsi.2004.12.007
WHO. 2006. Manual of diagnostic tests for aquatic animals. In: OIE Paris
Yuasa K, Koesharyani I, Roza D, Mori K, Katata M, Nakai T. 2002. Immune response of humpback grouper, Cromileptes altivelis (Valenciennes) injected with the recombinant coat protein of betanodavirus. Journal of Fish Diseases 25(1): 53–56. http://dx.doi.org/10.1046/j.1365-2761.2002.00325.x