Enhancement Glutathione Peroxidase Activity and α2-Macroglobulin Gene Expression of Macrobrachium rosenbergii Fed With Aqueous Morinda citrifolia Leaves Extract-Supplemented Diet

Authors

  • Atika Marisa Halim University of Brawijaya Malang
  • Arief Prajitno
  • Chin Chyuan Chang

DOI:

https://doi.org/10.22219/ijota.v1i1.4849

Abstract

Morinda citrifolia, known commercially as noni is often used for enhance immunity, these plant rich of phenolic compound with antioxidant properties. In the present study, Macrobrachium rosenbergii were fed with diets containing of aqueous M. citrifolia leaves extract (AMLE) at 0.6, 4 and 6 g kg-1. Glutathione peroxidase (GPx) activity was conducted to measure immune parameter, these parameter was evaluated before and after 7, 21, 35, 49 and 63 days of feeding trial. Immune gene expression of α2-macroglubulin (α2-M) was evaluated in this study. The results showed that after 63 days of feeding treatment, significantly increased in GPx activity. Gene expressions of α2-macroglubulin was significantly upregulated. These results recommend that administration of AMLE can be used as an immunostimulant and regulated immune response and immune gene expression in M. rosenbergii.

 

Keyword: Morinda citrifolia, Macrobrachium rosenbergii, GPx, α2-M

Downloads

Download data is not yet available.

References

Arizo MA, Beroncal RAG, Chua WAKT, Lim JJE, Rogando KCS and Maningas MBB. Immune response of Macrobrachium rosenbergii immersed in aqueous extract of Gracilaria edulis challenged with white spot syndrome virus. AACL Bioflux. 9 (2): 215-220.

Balasundram N, Sundram K, Samman S. 2006. Phenolic compounds in plants and agri-industrial by products: antioxidant activity, occurrence and potential uses. Food Chemistry. 99: 191-203.

Cheng W, J-C Chen. 1998. isolation and characterization of Enterococcus- like bacterium causing muscle necrosis and mortality in Macrobrachium rosenbergii in Taiwan. Disease of Aquatic Organisms. 34: 93-101.

Cheng W, Su-Mei Chen, Feng-I Wang, P-I. Hsu, C-H Liu, J-C. Chen. 2003. Effects of temperature, pH, salinity and ammonia on the phagocytic activity and clearance efficiency of giant fresh water prawn Macrobrachium rosenbergii to Lactococcus garvieae. Aquaculture. 219: 111-121.

Cheng W, C-H Liu, C-H Tsai, J-C Chen. 2005. Molecular cloning and characterization of a pattern recognition molecule, lipopolysaccharide- and β-1,3-glucan binding protein (LGBP) from the white shrimp Litopenaeus vannamei. Fish and Shellfish Immunology. 18: 297-310.

Govind P, Madhuri S, Mandloi AK. 2012. Immunostimulant Effect of Medicinal Plants on Fish. International Research Journal of Pharmacy. 3 (3): 112-114.

Hossain MA, Al-Raqmi KAS, Al-Mijizy ZH, Weli AM, Al-Riyami Q. 2013. Study of total pheol, flavonoids contents and phytochemical screening of various leaves crude extracts of locally grown Thymus vulgaris. Asian Pacific Journal of Tropical Biomedicine. 3 (9): 705-710.

Hsu JP, Liu CI. 1994. Studies on yeast infection in cultured giant freshwater prawn (Macrobrachium rosenbergii). Fish Disease Research .15:55-0.68.

Jayaraman, S.K., M.S. Manoharan, S. Illancheizan. 2008. antibacterial, antifungal and tumor cell suppression potential of Morinda citrifolia fruit extracts. International Journal of Integrative Bioloogy. 3 (1): 44-49.

Kumaran T, Devakumar D, Jayanthi J, Ragunathan MG. 2013. Stimulated immune response by Morinda tinctoria methanol leaf extract in a Vibrio parahaemolyticus infected fresh water crab, Oziotelphusa senex senex. International Journal Current Science. 5: 145-152.

Liu C-H, S-P Yeh, P-Y Hsu, Cheng W. 2007. Peroxinectin gene transcription of the giant freshwater prawn Macrobrachium rosenbergii under intrinsic, immunostimulant and chemotherapeutant influences. Fish and Shelfish Immunology. 22 (4): 408-417.

Liu, C-H, C-C Chang, Y-C Chiu, W Cheng, M-S. Yeh. 2011. Identification and cloning of transglutaminase from giant freshwater prawn Macrobrachium rosenbergii, and its transcription during pathogen infection and moulting. Fish and Shellfish Immunity. 31: 871-880.

Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-∆∆CT method. Methods. 25: 402-408.

Masuda M, Muraa K, Fukuhama A, Naruto S, Fujita T, Uwaya A, Isami F, Matsuda H. 2009. Inhibitory effects of constituents of Morinda citrifolia seeds on elastase and tyrosinase. Journal of Natural and Medicines. 0.63 (3): 20.67-273.

Nayak BS, Sandiford S, Maxwell A. 2009. Evaluation of the wound-healing activity of ethanolic extract of Morinda citrifolia leaf. Hindawi Publishing Corporation Scientific World Journal. 0.6(3): 0.631-0.630.6.

Nayak S, Mengi S. 2010. Immunostimulant activity of noni (Morinda citrifolia) on T and B lymphocytes. Pharmaceutical Biology. 48 (7): 724-731.

New MB. 2002. Farming Freshwater Prawns, A Manual For The Culture Of The Giant River Prawn (Macrobrachium rosenbergii). FAO, Fisheries Technical Paper, 428 p.

Nworu CS, Akah PA, Okoye FBC, Onwuakagba CJ, Okorafor OU, Esimone CO. 2012. Supplementation with aqueous leaf extract of Morinda lucida enhances immunorestoration and upregulates the expression of cytokines and imunostimulatory markers. Journal of Molecular and Cellular Immunology. 41 (8): 799-819.

Pham MA, Lee K-J, Lee B-J, Lim S-J, Kim S-S, Lee Y-D, Heo M-S, Lee K-W. 2006. Effects of Dietary Hizika fusiformis on growth and immune response in juvenile olive flounder (Paralichthys olivaceus). Asian-Australasian Journal of Animal Science. 19: 1769-1775.

Rattanavichai W, Cheng W. 2015. Dietary supplement of banana (Musa acuminata) peels hot-water extract to enhance the growth, anti-hypotermal stress, immunity and disease resistance of the giant freshwater prawn, Macrobrachium rosenbergii. Fish and Shellfish Immunology. 43: 415-420.

Rivera A, Cedillo L, Hernández F, Castillo V, Sánchez A, Castaǹeda D. 2012. Bioactive constituents in ethanolic extract leaves and fruit juisce of Morinda citrifolia. Annals of Biological Research. 3 (2): 1044-1049.

Usha R, Sashidharan S, Palaniswamy M. 2010. Antimicrobial activity of rarely known species, Morinda citrifoia L. Journal of Ethnobotanical Leaflets. 14: 300.6-311.

Wang, Wei-Na, Li B-S, Liu J-J, Shi L, Alam MJ, Su S-J, Wu J, Wang L, Wang An-Li. 2012. The respiratory burst activity and expression of catalase in white shrimp, Litopenaus vannamei, during long-term exposure to pH stress. Exotoxicology. 21: 1609-1616.

Zin ZM, Hamid AA, Osman A, Saari N. 2000.6. Antioxidative activities of chromatographic fractions obtained from root, fruit and leaf of Mengkudu (Morinda citrifolia L.). Food Chemistry. 94: 10.69-178.

Downloads

Published

04-08-2018

How to Cite

Halim, A. M., Prajitno, A., & Chang, C. C. (2018). Enhancement Glutathione Peroxidase Activity and α2-Macroglobulin Gene Expression of Macrobrachium rosenbergii Fed With Aqueous Morinda citrifolia Leaves Extract-Supplemented Diet. IJOTA (Indonesian Journal of Tropical Aquatic), 1(1), 9–16. https://doi.org/10.22219/ijota.v1i1.4849

Issue

Vol. 1 No. 1 (2018): August

Section

Articles

License

Copyright (c) 2018 Indonesian Journal of Tropical Aquatic

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

IJOTA (Indonesian Journal of Tropical Aquatic) allows readers to read, download, copy, distribute, print, search, or link to its articles' full texts and allows readers to use them for any other lawful purpose. The journal allows the author(s) to hold the copyright without restrictions. Finally, the journal allows the author(s) to retain publishing rights without restrictions

  • Authors are allowed to archive their submitted article in an open access repository
  • Authors are allowed to archive the final published article in an open access repository with an acknowledgment of its initial publication in this journal