Simple storage of sperm cells using combination of coconut and glycerol water towards motility and viability of Koi sperm (Cyprinus carpio)

Authors

  • Agista Tsani Untsa university of Muhammadiyah Malang
  • Ganjar Adhywirawan Sutarjo University of Muhammadiyah Malang
  • Riza Rahman Hakim University of Muhammadiyah Malang

DOI:

https://doi.org/10.22219/ijota.v2i1.7327

Keywords:

Cyprinus carpio, Motility, Sperm, Viability

Abstract

Fish sperm is a crucial part of fish reproduction, especially for Cyprinus carpio. This study aimed to determine the effect of coconut water and glycerol on the motility and viability of sperm koi fish (Cyprinus carpio) and the best treatment on sperm storage of Showa koi fish.This research was conducted for 45 days at Installation ofPunten Freshwater Cultivation - Malang. Thecompletely randomized design (CRD) used with five treatments and three replications. The treatment comes in 750µl coconut water, 500µl coconut water, and 250µl glycerol, 375µl coconut water, and 375µl glycerol, 250µl coconut water and 500µl glycerol, and 750µl glycerol (P1, P2, P3, P4, and P5, respectively). Briefly, the spermis stored at a low temperature (4℃). Observation of sperm motility used sperm cell dilution contained physiologicalNaCl, while the viability calculation used eosin 2% by the smear method. Motility and viability investigationwere employedwere carried out under a microscope with 400x magnification. The results revealed that the addition of coconut water and glycerol had a significant difference on the motility and viability of sperm koi fish.  It could survive 96 hours of storage, but the longer motility and viability would decrease. The P3 showed the best result compared to others. The P3 had an average motility scale of 96 - 5.00 and the average viability is 87.38%. Based on it, the treatment could increase the motility and viability of koi fish sperm.

 

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Author Biographies

Agista Tsani Untsa, university of Muhammadiyah Malang

Department of Aquaculture

Ganjar Adhywirawan Sutarjo, University of Muhammadiyah Malang

Fisheries Department of University of Muhammadiyah Malang

Riza Rahman Hakim, University of Muhammadiyah Malang

Fisheries Department of University of Muhammadiyah Malang

References

Arianti. 2013. Determination of time equilibration on freezing horse cement using skim milk diluent materials. Animal Reproduction. 9(3): 145–152.

Hidayaturrahmah. 2007. Waktu motilitas danviabilitas spermatozoa Ikan Mas (Cyprinus carpio L.) pada beberapa konsentrasi larutan fruktosa. Bioscientiae. 4(1): 9–18.

Hasan F. 2013. Pemberian Berbagai Jenis Madu Dengan Rasio Pengenceran Berbeda Setelah Masa Penyimpanan Terhadap Kualitas Sperma Ikan Patin Siam (Pangasius hypopthalamus). Repository IPB :Bogor.

Isnan F, Basuki, Susilowati. 2013. Penambahanair kelapadan gliserolpadapenyimpananspermaterhadapmotilitasdanfertilitasspermatozoa Ikan Mas (Cyprinus carpioL.). Journal of Aquaculture Management and Technology. 2(1): 51–65.

LeboeufB, Restall B, Salamon, S. 2000. Production and storage of goat semen for artificial insemination. Animal Reproduction Science. 62: 113–141.

Medeiros FC, Evans G, Maxwell WMC. 2002. Cryopreservation of stallion sperm using different amides. Theriogenology. 58: 273–276.

Novianto, Sudarno, Endang DM. 2014. Pengaruh perbedaan konsentrasi gliserol dalam susu skim kuning telur untuk prosespenyimpanan sperma beku terhadap motilitas danviabilitas spermatozoa ikan patin (Pangasius pangasius). Jurnal Ilmiah Perikanan dan Kelautan. 6(1): 35–43.

Sartoyo. 2005. Pengaruh Konsentrasi Dan Suhu Penyimpanan Terhadap Kualitas Sperma Ikan Mas Koki (Carassius auratus). Repository Unair :Surabaya.

Suswahyuningtyas. 2007. Pengaruh penambahan streptomycin dalam skimkuning telur sebagai pengencer terhadap kualitas semenikan mas (Cyprinus carpio L.). Jurnal Protein. 15(2): 145–152.

UdhardtU, Hesse S, Klemm D. 2005, Analytical investigation of baterialcellulose. Macromolecular Symposia. 223(1): 201–212.

Zairin M, Handayani S, Supriatna. 2005. Kualitas sperma Ikan Batak (Tor Soro) hasil kriopreservasi semen menggunakan dimetilsulfoksida (DMSO) dan gliserol. Jurnal Akuakultur Indonesia. 4(2): 145–151.

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Published

01-02-2019

How to Cite

Untsa, A. T., Sutarjo, G. A., & Hakim, R. R. (2019). Simple storage of sperm cells using combination of coconut and glycerol water towards motility and viability of Koi sperm (Cyprinus carpio). IJOTA (Indonesian Journal of Tropical Aquatic), 2(1), 25–32. https://doi.org/10.22219/ijota.v2i1.7327

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