Endophytic fungi of various medicinal plants collected from evergreen forest Baluran national park and its potential as laboratory manual for mycology course

Authors

  • Siti Murdiyah Jember University

DOI:

https://doi.org/10.22219/jpbi.v3i1.3977

Keywords:

endophytic fungi, laboratory manual, medicinal plants

Abstract

Endophytic fungi found on a variety of medicinal plants may express particular benefit. These fungi provide an alternative to overcome the progressive microbial resistance and as an effort to combat infectious diseases that became one of the leading causes of mortality. The main objective of this study was to isolate endophytic fungi from leaf samples of five medicinal plants species collected from evergreen forests Baluran National Park and its use as laboratory manual for Micology. Research findings showed there were 3 isolates of endophytic fungi isolated from 2 medicinal plants namely Kesambi (Schleicera oleosa) and Ketapang (Terminalia catappa). All three isolates formed sporangiophores as asexual reproductive structures, while the structure of sexual still undiscovered therefore its classification has not been determined. The validity tests also showed that the lab manual is feasible for use with the percentage achievement 85.37% and 88.56%.

Downloads

Download data is not yet available.

Author Biography

Siti Murdiyah, Jember University

Lecturer at Department of Biology Education,

Faculty of Teacher Training and Education

Jember University

References

Alexopoulos, C. J. & Mims, C. W. (1996). Introductory mycology. New York: John Wiley & Sons.

Anandha, K. & Sridhar, K. R. (2002). Diversity of endophytic fungi in the roots of mangrove species on the west coast of India. Can. J. Microbiol. 48: 871–878.

Arnold, M., Kyllo, R. & Robbins. (2003). Fungal endophytes limit pathogen damage in a tropical tree. PNAS, 100 (26), 15649–15654.

Azevedo, J. L., Macheroni, W., Pereira, J. O. & Araujo, W. L. (2000). Endophytic microorganisms: A review on insect control and recent advances on tropical plants. Electronic Journal of Biotechnology, 3 (1), Issue of April 15.

Bharathidasan & Panneerselvam, A. (2011). Isolation and identification of endophytic fungi from Avicennia marina in Ramanathapuram District, Karankadu, Tamilnadu, India. In European Journal of Experimental Biology, 1 (3), 31-36.

Ellis, D., Davis, S., Alexiou, H., Handke, R., & Bartley, R. (2007). Description of medical fungi. Australia: Mycology Units’ Women and Children Hospital.

El-Nagerabi, S., El-Shafie, A. E., & Al-Khanjari, S.S. (2013). Endophytic fungi associated with Ziziphus species and new records from mountainous area of Oman. BIODIVERSITAS, 14 (1), 10-16.

Hawksworth, D. L., & Crous, P.W. (2014). IMA fungus. The Global Mycologycal Journal, 5 (1), June 2014.

Hibbet, D., Binder, M., Bischoff, J., Blackwell, M., Cannon, P.F., Eriksson, O.E., Huhndorf, S., Timothy, J., Kirk, P.M., & Lucking, R., (2007). A higher-level phylogenetic classification of the Fungi. Mycological Research, 1 (11), 509 – 547.

Huang, W.Y. Cai, Y.Z. Xing, J. Corke, J.H. and Sun, M. (2007). A Potential Antioxidant Resource: Endophytic Fungi From Medicinal Plants. Econ Bot, 61:14–30.

Imamah, E. Q., Lestari, U. & Gofur, A. (2016). Developing booklet based on the research result of the effect of formalin-added-tofu to hepar histopatology of male mice Balb/C Strain. Jurnal Pendidikan Biologi Indonesia, 2(2), 102-108.

Kasi, Y. A., Posangi, J., Wowor, P. M. & Bara, R. (2015). Uji efek antibakteri jamur endofit daun mangrove Avicennia marina terhadap bakteri Uji Staphylococcus aureus dan Shigella dysenteriae. Jurnal e-Biomedik (eBm), 3 (1), Januari-April 2015.

Liang, H., Yongmei, X., Juan, C., Dawei, Z., Shunxing, G., & Chunlang, W. (2012). Antimicrobial activities of endophytic fungi isolated from Ophiopogon japonicus (Liliaceae). In BMC Complementary and Alternative Medicine 2012, 12:238.

Noverita, Fitria, D., & Sinaga, E. (2009). Isolasi dan uji aktifitas antibakteri jamur endofit dari daun dan rimpang Zingiber ottensii Val. Retrieved from https://www.researchgate.net/publication/235981709.

Radji, M. (2005). Peranan bioteknologi dan mikroba endofit dalam pengembangan obat herbal. Laboratorium Mikrobiologi dan Bioteknologi. Departemen Farmasi, FMIPA-UI. Majalah Ilmu Kefarmasian, 2 (3), 113-126.

Rante, H. Taebe, B. & Intan, S. (2013). Isolasi fungi endofit penghasil senyawa antimikroba dari daun cabai katokkon (Capsicum annuum L var. Chinensis) dan Profil KLT Bioautografi. Majalah Farmasi dan Farmakologi, 17 (2), 39 – 46.

Restiani, R. A., Suarsini, E. & Indriwati, SE. (2016). Test of fungi number of syzygium polyanthum bark simplisia for traditional medicine as disseminate material to the low economic communities in Malang. Jurnal Pendidikan Biologi Indonesia, 2 (3), 300-308.

Sia, E. D,. Marcon, J., Luvizotto, D. M., Quecine, M. C., Tsui, S., Pereira, J. O., & Azevedo, J. L. (2013). Endophytic fungi from the Amazonian plant Paullinia cupana and from Olea europaea isolated using cassava as an alternative starch media source. SpringerPlus, 2:579.

Simlai, A., Rai, A., Mishra, S., Mukherjee, K., & Roy, A. (2014). Antimicrobial and antioxidative activities in the bark exstract of Sonneratia caseolaris, a mangrove species. Journal of Experimental and Clinical Science. 13 (Augt), 997-1010.

Sudantha, I. M. 2009. Karakterisasi jamur saprofit dan potensinya untuk pengendalian jamur Fusarium oxysporum F. sp. Vanillae pada tanaman vanili. Agroteksos, 19 (3), 89-100.

Sugijanto, N. E., Indrayanto, G. Zaini, N. C. (2004). Isolasi dan determinasi berbagai jamur endofit dari tanaman Aglaia elliptica, Aglaia eusideroxylon, Aglaia odorata dan Aglaia odoratissima. Jurnal Penelitian Medika Eksakta, 5 (2).

Tejesvi & Pirttila. (2011). Potential of tree endophytes as sources for new drug compounds. In Pirttila dan Frank, A.C. (Eds.). Endophytes of Forest Trees Biology and Applications. New York: Springer-Dordrecht.

Wang, K. Shiwei, W. Bin, W. & Jiguang, W. (2014). Bioactive natural compounds from the mangrove endophytic fungi. Medicinal Chemistry, 14: 370-391.

Yunus, A. (2012). Isolasi fungi endofit dari tanaman Artemisia Annua L dan Mengetahui Kemampuannya dalam Menghasilkan Artemisinin. Retireved from http://eprints.uns.ac.id/id/eprint.

Downloads

Published

2017-03-31

Issue

Vol. 3 No. 1 (2017): MARCH

Section

ICT and Learning Media

License

Authors who publish with JPBI (Jurnal Pendidikan Biologi Indonesia) agree to the following terms:

  1. For all articles published in JPBI, copyright is retained by the authors. Authors give permission to the publisher to announce the work with conditions. When the manuscript is accepted for publication, the authors agree to automatic transfer of the publishing right to the publisher.
  2. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-ShareAlike 4.0 International License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
  3. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  4. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.