Kemiri Sunan Buds Induction (Reutealis trisperma (Blanco) Airy Shaw) For In Vitro in Ms Media with Different Concentrations of the Substance Growing Manager
DOI:
https://doi.org/10.22219/jtcst.v6i2.35568Keywords:
Fertilizer, Inpara, DosageAbstract
Need oil fuel in Indonesia has increased. Oil production reached only 410,000 barrels per day while the needs of up to 1 million barrels per day. One plant is capable of producing biofuels is Pecan sunan. Vegetative reproduction has been hampered by the limited number of obtained so that necessary research in vitro. This research aims to know the concentration of the substance growing balance sitokinin (BA, TDZ and kinetin) and Auxin (NAA) in Pecan sunan buds induces on the MS medium. Experimental design used was Complete Random Design simple. The treatment is the composition of the basic MS medium with medium that is combined with the astringent balance grow NAA (0.5 mg/l), BA (1-3 mg/l), TDZ (0.2 mg/l) and kinetin (2 mg/l). The result of the observation form buds, but callus changes color and size kalus. Before and after color callus subculture of potentially forming buds is the A9 (MS + 0.5 mg/l NAA + 3 mg/l BA + 2 mg/l kinetin). While kalus response tends to be good on the A5 is 0 MS media (MS + 0.5 mg/l NAA + 2 mg/l BA + 0.2 mg/l TDZ) and G media is A2 (MS + 0.5 mg/l NAA + 2 mg/l BA).
Downloads
References
Dhany, R.R. 2015. Ini Bukti Selisih Produksi Minyak RI dan Konsumsi BBM Makin Lebar. http://finance.detik.com/. diakses desember 2015
Gunawan, L.W. 1988. Teknik Kultur Jaringan. Bogor: Laboratorium Kultur Jaringan, PAU Bioteknologi, IPB.
George, E.F. And P. D. Sherrington. 1984. Plant Propagation By Tissue Culture. Exegetics Ltd,
Hardiyanto, A., Solichatun dan W. Mudyantini. 2004. Pengaruh Variasi Konsentrasi AsamNaftalen Asetat terhadap Pertumbuhan dan Kandungan Flavonoid Kalus Daun Dewa [Gynura procumbens (Lour) Merr.]. jurnal biofarmasi. Volume 2, Nomor 2, Halaman 69-74.
Kurniati, E., E. Mursyanti dan L. M. E. Purwijantiningsih. 2013. Induksi Kalus dan Penghasilan Capsaicin pada Variasi Kadar Nutrien MS dan Kombinasi Zat Pengatur Tumbuh. Naskah Publikasi. Fakultas Teknobiologi Universitas Atma Jaya Yogyakarta. Halaman 1-13.
Lestari, E.G. dan Mariska, I. 2003. Pengaruh Berbagai Formulasi Medium Terhadap Regenerasi Kalus Padi Indica. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman. 257.
Lestari, E.G., 2011. Peranan Zat Pengatur Tumbuh dalam Perbanyakan Tanaman melalui Kultur Jaringan. Jurnal Agro Biogen Volume 7, Nomor 1, Halaman 63-68.
Mok, MC & Mok, DW 1987, ‘Biologycal And Biochemical Effects Of Cytokinin-Active Phenilurea Derivates In Tissue Culture System’, Hort Sci., vol. 22, no. 6, pp.1194-201
Pranowo, D., M. Syakir, B. Prastowo, M. Herman, A. Aunillah, dan Sumanto. 2014. Pembuatan Biodiesel Dari Kemiri Sunan (Reutealis trisperma (Blanco) Airy Shaw) Dan Pemanfaatan Hasil Samping. Jakarta:IAARD Press. Halaman 13-95.
Rachmawati, purwito, a., wiendi, NMA., mattjik, na dan winarto, b. 2014. Perbanyakan Massa Anggrek Dendrobium Gradita 10. J. Hort. 24(3):196-209
Salisbury, F.B., dan C.W. Ross. 1995. Fisiologi tumbuhan. Jilid 1 Terjemahan Diah R. Lukman dan Sumaryo. ITB, Bandung.
Sumanto. 2005. Manfaat Tanaman Jarak Pagar Sebagai Tanaman Obat dan Bahan Baku Industri Minyak Biodisel yang Potensial. Warta Penelitian dan Pengembangan Tanaman Industri 11(1): 21-23.
Wattimena, G.A. 1991. Zat Pengatur Tumbuh. Bogor: PAU Bioteknologi IPB. Englang. pp. 3, 17, 119, 288.
Wijayani, Y., Solichatun dan W. Mudyantini. 2006. Pertumbuhan Tunas dan Struktur Anatomi Protocorm Like Body Anggrek Grammatophyllum scriptum (Lindl.) Bl. dengan Pemberian Kinetin dan NAA. Jurnal bioteknologi. Volume 4, nomor 2, halaman 33-40.
Wulandari, D., wan, S. dan yossilia. 2004. Respon Eksplan Daun Tanaman Jeruk Manis (Citrus sinensis L.) Secara In Vitro Akibat Pemberian NAA dan BA. Jurnal biogenesis vol. 1(1):21-25
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Duwi Risti Ningseh
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).