The Effect of IAA Concentration and Growing Media Composition on Banana Raja Bulu (Musa paradisiaca L.) Planlet Growth at Aclimatization Stage
Keywords:
husk charcoal, hormone, tissue cultureAbstract
Banana plants are an annua horticultural crops that has production capability without depend on the season and are widely cultivated in the tropic or subtropic areas. Tissue culture can produce good quality banana seedling in large quantities. Banana planlets from tissue culture require appropriate growing media and growth regulators such as auxin to stimulate their metabolic systems. The research aim to examine effect of IAA auxin hormone concentration, composition sand: compost: husk charcoal and its interactions on the growth of raja bulu banana plantlets. The research was conducted in July - September 2019 at Plant Physiology and Breeding Laboratory, Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang. The study used a complete randomized factorial design 4x2 with 5 replications. The first factor was IAA concentration (K) consists of 4 levels, K0: 0 ppm, K1: 30 ppm, K2: 60 ppm, K3: 90 ppm. The second factor was composition of growing media (M), M0: sand: compost: husk charcoal (1: 1: 1) and M1: sand: compost: husk charcoal (1: 1: 2). Data were analyzed by F test and proceeded by DMRT test at the level of 5%. The results showed that IAA significantly affected stem diameter, number of leaves, chlorophyll content and nitrate reductase activity of banana seedlings. The treatment of growing media significantly affected plant height and number of leaves. Concentration of 30 ppm IAA at all growing media increased significantly on stem diameter, number of leaves, chlorophyll content and nitrate reductase activity.
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