The Effect of Naftalene Acetic Acid and Benzyl Amino Purine on Growth of Potato Planlets In Vitro
DOI:
https://doi.org/10.22219/jtcst.v2i1.10418Keywords:
branch, contamination, nodes, organogenesis, rootsAbstract
The purpose of this research was to analyze the effect of Naftalene Acetic Acid (NAA) and Benzyl Amino Purine (BAP) on growth of potato plantlet (Solanum tuberosum L.). This research used factorial Completely Randomized Design (CRD). The first factor was the concentration levels of NAA (A1 = 0,5 ppm A2 = 1.5 ppm and A3 = 2.5 ppm). The second factor was the concentration levels of BAP (B1 = 1 ppm B2 = 2 ppm and B3 = 3 ppm). Parameter observed were number of nodes, number of branches, number of roots, percentage of life explants, and percentage of damaged explants. Data were analyzed with analysis of variance and continued by Least Significance Different (LSD). The results showed that NAA 0.5 ppm increased the number of nodes, branches, and roots compared to other treatments. The concentration of BAP 1 ppm increased the number of roots compared to other treatments. The concentration of NAA 0.5 ppm and BAP 1 ppm showed the best percentage of life explants (100 %) and showed the minimum percentage of damaged explants (0 %).Downloads
References
Asmono, S. L., & Sari V. K. (2016). Induksi kalus dari beberapa kultivar tanaman kentang (Solanum tuberosum L.) dataran medium secara in vitro menggunakan variasi konsentrasi 2,4-D. Jurnal Ilmiah Inovasi, 1(2), 116 – 121.
Badan Pusat Statistik. (2017). Produksi hortikultura buah dan sayuran di Indonesia 2014 – 2017. Jakarta: Badan Pusat Statistik.
Elfiani. (2013). Pengumbian in vitro kentang Granola. Jurnal Dinamika Pertanian, 28(1), 33 – 38.
Hidayah, P., Izzati M., & Parman S. (2017). Pertumbuhan dan produksi tanaman Kentang (Solanum tuberosum L. var. Granola) pada sistem budidaya yang berbeda. Jurnal Anatomi dan Fisiologi, 2(2), 218 – 225.
Husna, A. U., Siregar L. A. M., & Husni Y. (2014). Pertumbuhan dan perkembangan nodus kentang (Solanum tuberosum L.) akibat modifikasi konsentrasi sukrosa dan penambahan 2-Isopenteniladenina secara in vitro. Jurnal Online Agroekoteknologi, 2(3), 997 – 1003.
Isda, M. N., & Fatonah S. (2014). Induksi akar pada eksplan tunas Anggrek (Grammatophylum scriptum var. citrinum) secara in vitro pada media MS dengan penambahan NAA dan BAP. Jurnal Biologi, 7(2), 53 – 57.
Karjadi, A. K. (2016). Kultur jaringan dan mikropropagasi tanaman kentang (Solanum tuberosum L.). Jurnal Iptek Tanaman Sayuran, 25(8), 1 – 10.
Kumlay, A. M., & Ercisli S. (2015). Callus induction, shoot proliferation, and root regeneration of potato (Solanum tuberosum L.) stem node and leaf explants under long-day condition. Journal of Biotechnology and Biotechnological Equipment, 29(6), 1075 – 1084.
Lestari, F. W., Suminar E., & Mubarok S. (2018). Pengujian berbagai eksplan kentang (Solanum tuberosum L.) dengan penggunaan konsentrasi BAP dan NAA yang berbeda. Jurnal Agro, 5(1), 66 – 75.
Mastuti, R. (2017). Dasar-dasar Kultur Jaringan Tumbuhan. Malang: UB Press.
Moeini, M. J., Armin M., Asgharipour M. R., & Yazdi S. K. (2011). Effects of different plant growth regulators and potting mixes on micro-propagation and mini-tuberization of potato plantlets. Journal of Advances in Environmental Biology, 5(4), 631 – 638.
Munggarani, M., Suminar E., Nuraini A., & Mubarok S. (2018). Multiplikasi tunas meriklon kentang pada berbagai jenis dan konsentrasi sitokinin. Jurnal Agrologia, 7(2), 80 – 89.
Pratama, A. R., Sugiyono, Prayoga L., & Husni A. (2014). Upaya memacu pertumbuhan tunas mikro kentang kultivar Granola dengan jenis dan konsentrasi sitokinin berbeda. Jurnal Scripta Biologica, 1(3), 209 – 215.
Sagala, D., Tubur H. W., Jannah U. F., & Sinath C. (2012). Pengaruh BAP terhadap pembentukan dan pembesaran umbi mikro kentang kultivar Granola. Jurnal Agroqua, 10(1), 5 -12.
Setiawati, T., Zahra A., Budiono R., & Nurzaman M. (2018). Perbanyakan in vitro tanaman kentang (Solanum tuberosum [L.] cv. Granola) dengan penambahan meta-topolin pada media modifikasi MS (Murashige & Skoog). Jurnal Metamorfosa, 5(1), 44 – 50.
Sukmadjaja, D. (2014). Pengadaan Benih Tanaman melalui Teknik Kultur Jaringan. Bogor: Penerbit Akademia.
Viola, Y. R. N., Roviq M., & Wardiyati T. (2017). Pengaruh konsentrasi BA terhadap pembentukan embrio somatik pada tanaman kentang (Solanum tuberosum L.) secara in vitro. Journal of Agricultural Science Plantropica, 2(1), 10 – 17.
Wartina, R. (2011). Pengaruh NAA dan BAP terhadap regenerasi kalus kentang (Solanum tuberosum L.) hasil induksi mutasi ethyl methane sulphonate (EMS). Jurnal Tanaman Hortikultura, 2(1), 1 – 9.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2020 Nurmufiidah et al.
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
